Whole-genome shotgun assembly and analysis of the genome of Fugu rubripes.
نویسندگان
چکیده
The compact genome of Fugu rubripes has been sequenced to over 95% coverage, and more than 80% of the assembly is in multigene-sized scaffolds. In this 365-megabase vertebrate genome, repetitive DNA accounts for less than one-sixth of the sequence, and gene loci occupy about one-third of the genome. As with the human genome, gene loci are not evenly distributed, but are clustered into sparse and dense regions. Some "giant" genes were observed that had average coding sequence sizes but were spread over genomic lengths significantly larger than those of their human orthologs. Although three-quarters of predicted human proteins have a strong match to Fugu, approximately a quarter of the human proteins had highly diverged from or had no pufferfish homologs, highlighting the extent of protein evolution in the 450 million years since teleosts and mammals diverged. Conserved linkages between Fugu and human genes indicate the preservation of chromosomal segments from the common vertebrate ancestor, but with considerable scrambling of gene order.
منابع مشابه
Fugu rubripes Whole - Genome Shotgun Assembly and Analysis of the Genome
, 1301 (2002); 297 Science et al. Samuel Aparicio Fugu rubripes Whole-Genome Shotgun Assembly and Analysis of the Genome of This copy is for your personal, non-commercial use only. clicking here. colleagues, clients, or customers by , you can order high-quality copies for your If you wish to distribute this article to others here. following the guidelines can be obtained by Permission to repub...
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ورودعنوان ژورنال:
- Science
دوره 297 5585 شماره
صفحات -
تاریخ انتشار 2002